Mention a common vector used for amplification
of DNA
fragments during Sequencing
of human
genom
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Today, new plasmid vectors based on the naturally occurring F plasmid of E. coli are used to clone DNA fragments of 300,000 to 1 million nucleotide pairs. Unlike smaller bacterial plasmids, the F plasmid—and its derivative, the bacterial artificial chromosome (BAC)—is present in only one or two copies per E. coli cell.
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