Mention the apparatus required to conduct the activity Rhizobium
Answers
(a) Recognizing legumes and identifying them in the field.
A suitable botanical key describing the identification of
legumes.
(b) Recovering nodules in the field.
Refrigerator
Spade
Sieve
Running water
Plastic bags for plants, smaller bags for seeds
(c) Preserving nodules
Refrigerator
Collection vial
Nodules from (b)
(d) Examining nodules and bacteroids
Microscope
Bunsen burner
Microscope slides, cover slips, mounting fluid
Razor blade, inoculation loopDistilled water
Carbol fuchsin stain
Rhizobial cultures
Nodulated plants from (b)
(e) Isolating rhizobia from a nodule
Refrigerator
Scissors, forceps, inoculation loop, isolation needle
Scalpel
Sterile Petri dishes
Sterile test tubes, glass rods
Erlenmeyer flask 125 ml (optional), small beaker
Bunsen burner
Sterile Pasteur pipettes
Sterile filter paper (cut into small pieces)
Running water, sterile water, ethanol or isopropanol
Sodium hypochlorite solution, 3% (may be made from
commercial bleach)
Plates of plain YMA, plates of YMA + Congo Red and YMA +
BTB
Liquid YMA (50°C)
Nodulated plants from (b)
Desiccated nodules
f) Performing the presumptive test
it will be helpful it can explain all the requirements for the apparatus for conducting the experiment of rhizobium.