mention the benefits from Pollen culture
Answers
Answered by
1
Pollen culture is a technique in which haploid plants are obtained from isolated pollen grains while in anther culture those are obtained from pollens, by placing anthers on a suitable, synthetic culture medium.
Answered by
1
Meaning of Pollen Culture:
Microspore or the immature pollen can be used as the explant to get the haploid plants directly. For pollen or microspore culture the flower buds are collected, surface sterilized and the anther lobes are dissected out from the flower buds as before. Then the anther lobes are squeezed with the help of a scalpel within a tube or small beaker to collect the microspore or pollen in nutrient media.
Then the anther tissue debris is removed by filtering the suspension through a nylon sieve with a diameter slightly larger than the pollen size (40µ-100µ) allowing the microspore only to pass through it.
Microspore or the immature pollen can be used as the explant to get the haploid plants directly. For pollen or microspore culture the flower buds are collected, surface sterilized and the anther lobes are dissected out from the flower buds as before. Then the anther lobes are squeezed with the help of a scalpel within a tube or small beaker to collect the microspore or pollen in nutrient media.
Then the anther tissue debris is removed by filtering the suspension through a nylon sieve with a diameter slightly larger than the pollen size (40µ-100µ) allowing the microspore only to pass through it.
abhisharma5011:
yaa bro
Similar questions