Question

mention the use of chromatography

Answer 1

Chromatography is a versatile separation technique widely usedto obtain pure compounds from mixtures. All chromatography can techniques depend on a stationary phase, usually a finely divided solid or coated solid, that a mobile phase, usually a gas or liquid, moves through.Basics of Chromatography

In any chromatographic technique, a stationary phase usually a solid, thick liquid, or bonded coating that stays fixed in one place, and a mobile phase or eluent (usually a liquid or gas) that moves through it or across it.

A sample to be separated, when placed on the stationary phase, will gradually move along in the same direction as the mobile phase. If a sample compound (or analyte) has no interaction with the stationary phase, it will run right through and come out of the system (elute) at the same rate as the mobile phase. On the other hand, if an analyte has no interaction with the mobile phase, it will stick directly to the stationary phase and never elute. Neither of these are good outcomes.

In a well-designed chromatography process, the chemist will choose stationary and mobile phases that will both have at least some interaction with the analytes. Any individual sample molecule will interact first with one phase and then the other, back and forth repeatedly, but the fraction of each analyte overall in each phase will remain constant. This distribution ratio among the selected phases must differ for each analyte in order for them to separate. (Compounds will not separate chromatographically if they have the same distribution ratio on a particular system.)

Answer 2

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