methodology of tissue culture
Answers
Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation
Answer:
Tissue Culture:
The procedure in which a small tissue pieces or organs is removed from a donor plant and cultured aseptically on a nutrient medium is called Tissue Culture. This production is also known as micropropagation technique. The synthetic medium contains all the nutrients and hormones which are required for the growth.
A cell or tissue is transferred into suitable synthetic medium under sterile conditions. The tissue then develops into a fast growing cellular mass called callus. The callus is transferred to another medium for growth and differentiation that forms plantlets. The plantlets can be transplanted into soil or pots where they can be grown to maturity.
Tissue culture technique is being popularly used in agriculture and horticulture. It is used for production of ornamental plants like Orchids, Dahlia and Carnation. Other examples are Banana, Fig, Lemon, Potatoes, etc.