Question

Methods employed for detection of virus growth in cell culture

Answer 1

Answer:

Virus isolation in cell cultures, immunofluorescence-based assay and molecular techniques to determine nucleic acid, have all been used successfully to detect and identify the viruses. On the other hand, the most commonly used methods to quantify viruses can be subdivided into three broader categories – techniques measuring viral infectivity (viral plaque assay, TCID50 and immunofluorescence foci assay), those that examine viral nucleic acid and protein.