Methods of introduction of recombination dna into mammalian cells
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There are several methods available for introducing genes into the mammalian cells.
It consists of chromosome-mediated, DNA-mediated gene transfer and cell hybridization.
When it comes to DNA-mediated transfer, it is achieved by the indirect methods or direct microinjection methods.
The DNA actually enters nucleus as well as is expressed properly in a high amount of the cells transiently.
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