Question

Methods of transformation transfection biolistic of insertion of dna into living cells

Answer 1

Answer:

Explanation:

Biolistic infection is the type of infection done by use of gene gun in which the targeted cell or genome is bombarded with a DNA which must be gold plated.  

The cells which gets penetrated by gold particles are absolutely transfected.

The infection is required by a vector to be performed the vectors help the DNA of interest to be inserted in it to be physically transferred and then transformed into the required genome.

1. This physical vector gene transfer is done by screening of the recombinant.
2. Insertional inactivation or the blue white selection.
3. Expression of desired gene by an expression vector.
4. At last the scaling up from small scale production to large scale production done in bioreactors.