Question

Methods to investigate protein protein interaction in vivo protein-protein​

Answer 1

Answer:

The in vitro methods in PPI detection are tandem affinity purification, affinity chromatography, coimmunoprecipitation, protein arrays, protein fragment complementation, phage display, X-ray crystallography, and NMR spectroscopy. In in vivo techniques, a given procedure is performed on the whole living organism itself.

Explanation:

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Answer 2

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Characterizing protein–protein interactions through methods such as co-immunoprecipitation (co-IP), pull-down assays, crosslinking, label transfer, and far–western blot analysis is critical to understand protein function and the biology of the cell.