Question

Methods to investigate protein protein interaction in vivo protein-protein

Answer 1

The increase in the polarization of the fluorescence upon binding of the labeled protein to its binding partner can be used to calculate the binding affinity. ... Fluorescence resonance energy transfer (FRET) is a common technique when observing the interactions of only two different proteins.

Answer 2

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Characterizing protein–protein interactions through methods such as co-immunoprecipitation (co-IP), pull-down assays, crosslinking, label transfer, and far–western blot analysis is critical to understand protein function and the biology of the cell.