Question

Modified baermann technique for nematode extraction

Answer 1

efficient recovery of the plant‐parasitic nematodes from the plant or soil sample. The easiest and most simple method is to submerge a plant sample in water in a Petri dish and directly select the nematodes for further identification using the microscope. Although this procedure can provide results in a very short time, it is only suitable for small samples, cannot be standardized and its overall efficacy is low. For soil samples it is not possible to observe nematodes directly due to the dense cloud of soil particles. For routine diagnostics, such as in the case of regulated plant pathogens, standardized methods are required that provides high nematode recovery under varying sample conditions. At the same time, those methods should allow only minimal individual failure and results should be reproducible. Over past years, many methods have undergone test performance studies nationally and internationally, but only few results have been published (e.g. Müller, 1983; Ladeveze &