Question

molecular hybridization different examples used in different diseses article

Answer 1

Hybridization (of nucleic acids) is a technique in which single-stranded nucleic acids are allowed to interact to form complexes, or hybrids with sufficiently similar complementary sequences. This technique allows the detection of specific sequences or may be used to assess the degree of sequence identity. Hybridization may be carried out in solution or more commonly on a solid-phase support, e.g., nitrocellulose paper. The hybrid of interest is often identified with a radioactively, or alternatively labeled nucleic acid probe or by digestion with an enzyme that specifically attacks single-stranded nucleic acids. Hybridization can be performed with combinations of DNA–DNA (heat-denatured to produce single strands), DNA–RNA, or RNA–RNA molecules. In situ hybridization of labeled nucleic acids with prepared cells or tissue sections is used to identify specific transcription or to locate genes on specific chromosomes (e.g., fluorescence in situ hybridization, FISH).