Question

Mr Yasir was performing microscopy of urine. He was having issue with the focusing of microscopic field. His fellow Mr. Ali told him about a technique known Köhler Technique. If you have any idea about this technique, write down in a proper detailed manner.
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Answer 1

Answer:

Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image. Köhler illumination is the predominant technique for sample illumination in modern scientific light microscopy.