my question is that in the experiment to show that chlorophyll is necessary for the process of photosynthesis why is the plant firstly destarched then again kept in sunlight for almost 6 hours? does the plant not go through the process of photosynthesis during these 6 hours time and if it does then it might have stored starch again then what was the need of destarching it at first?
Answers
Answer:
1. A different leaves plant is taken for this experiment . A different coloured leaf is a leaf in which different patches of colors are present in a Geranium plant.
2. The place where green chlorophyll pigment is present while at the other color patches present in the leaf contain other pigments but not chlorophyll.
before starting the experiment an important step has to be taken is known as De-starching. De-starching is a step in which the starch present in the leaves of the plant will be removed by keeping the variegated leaves plant in a dark room or closed cup-board for about 48 hours.
4. This step is done to ensure that previously synthesized starch in the leaves get consumed up because photosynthesis doesn't occur at that time as the plant is kept in dark place.
5.After De-starching step the variegated leaf plant is kept in bright sunlight for about 6 hours. After this one of the variegated leaf is plucked out from the experimental plant.
6. Then Iodine test is conducted for this experimental leaf to find out whether the leaf prepared starch through photosynthesis or not.