n a restriction enzyme digestion experiment, a DNA fragment of
I kilobases length is used, This fragment harbors three 5-GAATTC-33 restriction sites. After the restriction digestion of this fragment with Eco R, 3 DNA fragments coresponding to 500, 300 & 100 base pairs are observed in the agarose gel electrophoresis (AGE).
Predict the positions of the restriction site (GAATTC) in the undigested 1 kb- fragment which will result in the observed bands in AGE. (1) 100, 300 and 500 base pairs (2) 100, 200 and 500 base pairs (3) 200 and 500 base pairs (4) 100, 300 and 800 base pairs
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