Question

n preparing her immunology lab, an instructor purified IgG antibodies that are specific to sheep red blood cells (SRBCs)and digested some of the antibodies into Fab, Fc, and F(ab')2fragments. She placed each preparation in a separate tube, labeled the tubes with a water-soluble marker, and left them in an ice bucket. When the instructor returned, she discovered that the labels had smeared and were unreadable. Determined to salvage the antibodies, she relabeled the tubes 1, 2, 3, and 4 and proceeded. Based on the test results described below, indicate which preparation was contained in each tube and explain why you so identified the contents.

a. The preparation in tube 1 agglutinated SRBCs but did not lyse them in the presence of complement.

b. The preparation in tube 2 did not agglutinate SRBCs or lyse them in the presence of complement. However, when this preparation was added to SRBCs before the addition of whole anti-SRBC, it prevented agglutination of the cells by the whole anti-SRBC antiserum.

c. The preparation in tube 3 agglutinated SRBCs and also lysed the cells in the presence of complement.

d. The preparation in tube 4 did not agglutinate or lyse SRBCs and did not inhibit agglutination of SRBCs by whole anti-SRBC antiserum.

Answer 1

Explanation:

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