name any three tools for accomplishing the takes od recombinant dna technology mention the function of each tool
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Also, mention the functions of each tool.
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Key tools in rDNA technologyFunctions(b) Gel electrophoresis(b) Separating the desired DNA fragments.(c) Ligase enzyme(c) Creating recombinant DNA molecule.(d) DNA delivery system(d) To introduce gene in the host.
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1.Restriction endonucleases: - for cutting the desired DNA at desired places
2.Gel electrophoresis: - for separating the desired DNA fragments
3. Ligase enzyme: - for creating recombinant DNA molecule.
4.DNA delivery system:- like electroporation, microinjection, gene gun method.
5.Competent host (usually bacteria/yeast):- to take up recombinant DNA.
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