Name some recobinant proteins that are synthesied by insect cell culture notes
Answers
Insect cells offer high levels of protein expression with posttranslational modification approaching that of mammalian cells, ease of scale-up, and simplified cell growth that can be readily adapted to high-density suspension culture for large-scale expression. Most of the posttranslational modification pathways present in mammalian systems also occur in insect cells, allowing the production of recombinant protein that is more antigenically, immunogenically, and functionally similar to the native mammalian protein than if expressed in yeast or other eukaryotes. Baculovirus expression systems are powerful and versatile delivery and expression vehicles for producing high levels of recombinant protein expression in insect cells. Expression levels up to 500 mg/L have been reported using the baculovirus expression system.