Name the different type of chomatograph and explain the
Answers
Answer:
Explanation:
Some materials appear homogenous, but are actually a
combination of substances. For example, green plants contain a
mixture of different pigments. In addition, the black ink in the pens
that are used in this experiment is a mixture of different colored
materials. In many instances, we can separate these materials by
dissolving them in an appropriate liquid and allowing them to
move through an absorbent matrix, like paper.
Chromatography is a method used by scientists for separating
organic and inorganic compounds so that they can be analyzed and
studied. By analyzing a compound, a scientist can figure out what
makes up that compound. Chromatography is a great physical
method for observing mixtures and solvents.
The word chromatography means "color writing" which is a way
that a chemist can test liquid mixtures. While studying the coloring
materials in plant life, a Russian botanist invented chromatography
in 1903. His name was M.S. Tswett.
Chromatography is such an important technique that two nobel
prizes have been awarded to chromatographers. Over 60% of
chemical analysis worldwide is currently done with
chromatography or a variation thereon.
Chromatography is used in many different ways. Some people use
chromatography to find out what is in a solid or a liquid. It is also
used to determine what unknown substances are. The Police,
F.B.I., and other detectives use chromatography when trying to
solve a crime. It is also used to determine the presence of cocaine
in urine, alcohol in blood, PCB's in fish, and lead in water.
Chromatography is used by many different people in many
different ways.
Chromatography is based on differential migration. The solutes in
a mobile phase go through a stationary phase. Solutes with a
greater affinity for the mobile phase will spend more time in this
phase than the solutes that prefer the stationary phase. As the
solutes move through the stationary phase they separate. This is
called chromatographic development.
How it works
In all chromatography there is a mobile phase and a stationary
phase. The stationary phase is the phase that doesn't move and the
mobile phase is the phase that does move. The mobile phase moves
through the stationary phase picking up the compounds to be
tested. As the mobile phase continues to travel through the
stationary phase it takes the compounds with it. At different points
in the stationary phase the different components of the compound
are going to be absorbed and are going to stop moving with the
mobile phase. This is how the results of any chromatography are
gotten, from the point at which the different components of the
compound stop moving and separate from the other components.
In paper and thin-layer chromatography the mobile phase is the
solvent. The stationary phase in paper chromatography is the strip
or piece of paper that is placed in the solvent. In thin-layer
chromatography the stationary phase is the thin-layer cell. Both
these kinds of chromatography use capillary action to move the
solvent through the stationary phase.
W
Answer:
Chromatography is a versatile separation technique widely used to obtain pure compounds from mixtures. ... The five main types of chromatography include thin layer chromatography, gas chromatography, high-performance liquid chromatography, size exclusion chromatography, and affinity chromatography.