Question

name the factor RNA polymerase enzyme which recognises the start and termination signals on DNA for transcription process in prokaryote(becteria)​

Answer 1

Answer:

MRNA is the factor.

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Answer 2

Answer:

Hi Mate...

described earlier, transcription relies on the complementary pairing of bases. The two strands of the double helix separate locally, and one of the separated strands acts as a template. Next, free nucleotides are aligned on the DNA template by their complementary bases in the template. The free ribonucleotide A aligns with T in the DNA, G with C, C with G, and U with A. The process is catalyzed by the enzyme RNA polymerase, which attaches and moves along the DNA adding ribonucleotides in the growing RNA as shown in Figure 10-6a. Hence, already we see the two principles of base complementarity and binding proteins (in this case, the RNA polymerase) in action.

Figure 10-6. Transcription of two genes.

Figure 10-6

Transcription of two genes. (a) RNA polymerase moves from the 3′ end of the template strand, creating an RNA strand that grows in a 5′ → 3′ direction (because it must be antiparallel to the template strand). (more...)

RNA growth is always in the 5′ → 3′ direction: in other words, nucleotides are always added at a 3′ growing tip, as shown in Figure 10-6b. Because of the antiparallel nature of the nucleotide pairing, the fact that RNA is synthesized 5′ → 3′ means that the template strand must be oriented 3′ → 5′.

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RNA polymerase

In most prokaryotes, a single RNA polymerase species transcribes all types of RNA. Figure 10-7 shows the structure of RNA polymerase from E. coli. We can see that the enzyme consists of four different subunit types. The beta (β) subunit has a molecular weight of 150,000, beta prime (β′) 160,000, alpha (α) 40,000, and sigma (σ) 70,000. The σ subunit can dissociate from the rest of the complex, leaving the core enzyme. The complete enzyme with σ is termed the RNA polymerase holoenzyme and is necessary for correct initiation of transcription, whereas the core enzyme can continue transcription after initiation.

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