Question

Name the factor that effect the binding of O2 to haemoglobin to form oxyhaemoglobin in the tissue

Answer 1

$\mathfrak{\huge{\red{\underline{\underline{ANSWER :}}}}}$

✍️ There are several important factors that affect the affinity of hemoglobin to oxygen as therefore affect the oxygen-hemoglobin dissociation curve.

✍️ These factors include the (1) pH (2) temperature (3) carbon dioxide (4) 2,3-BPG and (5) carbon monoxide.

Hope It Will Help.❤️

Answer 2

Transport of Oxygen:

Theoretically,

• About 97% of oxygen is transported by RBCs in the form of oxyhaemoglobin
• The rest 3% is carried in a dissolved state via the plasma

General information about the transport of oxygen:

1. O₂ binds reversibly with haemoglobin to form oxyhaemoglobin
2. Each haemoglobin carries 4 molecules of O₂
3. In a normal person, about 15 gm of Hb is present per 100 mL of blood.
   ∴ 1 gm of Hb can bind with 1.34 mL of O₂.
4. Under normal conditions,
   5 mL of O₂ is delivered to the tissues by 100 mL of blood, and,
   During exercise, 15 mL of O₂ is delivered to the tissues by 100 mL of blood.

Oxygen dissociation Curve:

Refer to the diagram below for a better understanding.

P₅₀ value: The P₅₀ value is defined as the point where saturation of haemoglobin with oxygen is 50%, at a partial pressure of oxygen being 27 mm Hg.
P₅₀ ∝ (1/affinity)

Thus, the P₅₀ value can give us a direct estimation of the change in the degree of binding of oxygen with haemoglobin.

Factors that affect the P₅₀ values:

1. The partial pressure of CO₂: When $P_{CO_{2} }$ ↑, oxygen affinity ↓ and vice-versa.
2. The partial pressure of H: When $P_{H}$ ↓, oxygen affinity ↓ and vice-versa.
3. The concentration of H⁺ ion: When [H⁺] ↓, the oxygen affinity ↑ and vice-versa.
4. Temperature: When temperature ↓, the oxygen affinity ↑ and vice-versa.
5. The concentration of 2,3-BPGA [ 2,3-Bisphosphate Glyceric acid]: When 2,3-BPGA ↓, the oxygen affinity ↑ and vice-versa.

#SPJ3