Question

Name the method and chemical used by Camilo Golgi to study Golgi Apparatus ?​

Answer 1

Answer:

The Golgi staining technique, also called the black reaction after the stain's color, was developed in the 1870s and 1880s in Italy to make brain cells (neurons) visible under the microscope. Camillo Golgi developed the technique while working with nervous tissue, which required Golgi to examine cell structure under the microscope. Golgi improved upon existing methods of staining, enabling scientists to view entire neurons for the first time and changing the way people discussed the development and composition of the brain's cells. Into the twenty-fist century, Golgi's staining method continued to inform research on the nervous system, particularly regarding embryonic development.

Golgi began publishing research about the nervous system and its cellular structure between 1870 and 1872, while working in the laboratory of Giulio Bizzozero at the Institute of General Pathology at the University of Pavia in Pavia, Italy. Afterward, Golgi became chief doctor at the Pio Luogo degli Incurabili (Hospital for the Chronically Sick) in Abbiategrasso, Italy. He built a laboratory in the kitchen of the hospital, where he developed a new technique to stain neural tissues. The staining method of his time involved staining an organic tissue with red carmine or black hematoxylin and exposing it to a reagent, like potassium dichromate or chromic acid, that hardened the individual neurons. Scientists then isolated individual cells by separating them under a microscope with needles.

Explanation:

Answer 2

Golgi's method is a silver staining technique that is used to visualize nervous tissue under light microscopy. The method was discovered by Camillo Golgi, an Italian physician and scientist, who published the first picture made with the technique in 1873.