Question

name the stain and mounting chemical used during the temperory mount of leaf peel why do we use these two

Answer 1

we should use saffranin solution as it strains the cells in red colour
red can be easily identified

Answer 2

The correct answer is - Saffranin and glycerin.
After the leaf is plucked from a plant, the small portion of the lower part of the leaf is peeled off using a forceps and is placed in a watch glass that contains water. A few drops of the Safranin stain is added to the watch glass. The leaf is allowed to rest in this Safranin -water solution for 2-3 mins after which it is placed on a cleaned glass slide.
A few drops of glycerin is then added on this leaf portion and a clean coverslip is placed carefully on top of it. The excess safranin stain and glycerin can be removed by using blotting paper. This slide is then observed for stomata under a low and high-magnifying microscope.
The stomata can be observed as bean-shaped cells that contain the chloroplasts and a nucleus.

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