Question

Name three stains that can be used for negative staining

Answer 1

negative stain is used to determine cell size and arrangement.
1 nigrosin
2 india ink
3 eosin.

Answer 2

The stains that can be used for negative staining are:

• Ammonium molybdate
• Osmium tetroxide
• Osmium ferricyanide
• Auroglucothionate
• Uranyl acetate

• Uranyl formate
• Phosphotungstic acid.

• In microscopy, negative staining is an established method, which is used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid.
• In this technique, the background is stained, leaving the actual specimen untouched, and thus it is visible.
• This contradicts with positive staining, in which the actual specimen is stained.
• The choice of negative stain in electron microscopy is very important.
• An ancient study of plant viruses using negatively stained leaf dips from a diseased plant showed only spherical viruses with one stain and only rod-shaped viruses with another.
• The verified conclusion was that this plant suffered from a mixed infection by two different viruses.

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