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Crossing over is essential for the normal segregation of chromosomes during meiosis. Crossing over also accounts for genetic variation, because due to the swapping of genetic material during crossing over, the chromatids held together by the centromere are no longer identical.
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Crossing over occurs during prophase I of meiosis before tetrads are aligned along the equator in metaphase I. By meiosis II, only sister chromatids remain and homologous chromosomes have been moved to separate cells. Recall that the point of crossing over is to increase genetic diversity.
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