Nitrogen fixation by the microorganisms can be detected by adopting the approach of
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Nitrogen fixation is a process by which molecular nitrogen in the air is converted into ammonia or related nitrogenous compounds in soil. Atmospheric nitrogen is molecular dinitrogen, a relatively nonreactive molecule that is metabolically useless to all but a few microorganisms.
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The fixation of atmospheric nitrogen results from symbiosis between leguminous crops and Rhizobium bacteria, which on one hand form nodules on the plant roots and fix atmospheric nitrogen and on the other hand utilize carbohydrates and minerals from the host plant.
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