non diffusible costituent present in blood
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The transformation of the Folin-Wu method for the preparation
of protein-free blood filtrates into a method giving protein-free
extracts from unlaked blood (1) involves experimentally only the
simplest sort of modification, but produces results of far reaching
consequences. There will be necessarily a sharp break between
the hospital records or the past published records of blood analyses
based on aqueous extracts from laked whole blood and corresponding records obtained from unlaked blood. But the conclusion
seems inescapable that for nearly every kind of metabolism study,
it must be obscuring and misleading to include in the analyses of
blood as a transportation system, an unknown mixture of residues
obtained from the destroyed cellular elements which have no direct connection with the circulating waste products or food
products.
The disadvantage arising from a loss of continuity between past
records and new ones made on unlaked blood should be partly
compensated for by the fact that in the future there should be less
need for revisions of methods.
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