Biology, asked by sarika1929, 1 year ago

note in brief :\huge\star\mathfrak\red{{micrografting:-}}

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Answered by Anonymous
1

\huge\star\mathfrak\pink{{Answer:-}}

\huge\star\mathfrak\blue{{MICROGRAFTING-}}

It is difficult to regenerate complete plants from meristem inwoody species like most of fruit and forest plants, thus as an alternative micrograftingis done to produce virus-free plants. The various steps in micrografting includescion preparation, rootstock preparation, in vitro grafting and acclimatization/hardening of the plants. The in vitro raised nucellar seedlings are used as rootstocks.The scion (meristem 0.1-0.4 mm) is obtained either from young growth of fieldgrown trees, defoliated glasshouse grown plants, or in vitro proliferated nodalsegments obtained from mature trees. The grafting is done with the help ofstereomicroscope, under aseptic conditions. Several viruses have been eliminatedvia micrografting in fruit plants such as Citrus Tristeza Virus, Peach latent mosaicviroid, Pear Vein Yellow virus.

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