Question

Note on oxidation of pyruvate​

Answer 1

Pyruvate is produced by glycolysis in the cytoplasm, but pyruvate oxidation takes place in the mitochondrial matrix (in eukaryotes). ... A carboxyl group is removed from pyruvate and released as carbon dioxide. The two-carbon molecule from the first step is oxidized, and NAD+ accepts the electrons to form NADH

Explanation:

Answer 2

Oxidation of pyruvate coincides with the removal of CO2 from the carboxylic group (-COOH) of pyruvate, thus it is also called oxidative decarboxylation of pyruvate.

Glycolysis results in the formation of two molecules of pyruvate that contains plenty of extractable energy. The following step is the oxidation of pyruvate, which lands up with the formation of two molecules of acetyl CoA. Simultaneously, two carbon atoms are released as CO2 out of the six carbon atoms originally present in glucose (C6H12O6). Two molecules of NADH are generated from two NAD+.

The pyruvate oxidation steps are administered by an enzyme complex called pyruvate dehydrogenase complex (PDC). In prokaryotes, pyruvate oxidation takes place within the cytoplasm. But in eukaryotes, it takes place within the matrix of the mitochondrion. Pyruvate enters the mitochondria across an inner membrane and arrives at the matrix (the innermost compartment of the mitochondrion).

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