note on sub culturing of monolayer culture
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Subculture (or passage) refers to the transfer of cells from one culture vessel to another culture vessel. Subculture usually (not always) involves the subdivision of proliferating cells that enables the propagation of a cell line. There are two types of subcultures-monolayer subculture and suspension subculture.
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Sub culturing is the method of transferring microorganisms from one growth container to another, thereby offering them with fresh supply of nutrients either in a solid or a liquid medium.So the basic objective of this test is to prepare subculture of bacteria and fungus.
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