Question

Nucleotides are always added to the growing dna strand at the 3 end, at which the dna has a free

Answer 1

Nucleotides are added one by one and the growing strand of DNA remains attached to the glass beads until the synthesis is complete Modern DNA synthesizers are also usually capable of adding fluorescent dyes, biotin, or other groups used in labeling and detection of DNA. DNA is made only with chemical reagents; the process requires some special modifications nor necessary if biological enzymes were to manufacture the DNA. The first problem is that each deoxynucleotide has two hydroxyl groups ...

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