Question

nurse tissue technique is applied in..which culture?

Answer 1

nurse tissue technique is applied in
This method is actually a modification of petridish plating method and the paper raft nur­se culture method. In this method, single cells are plated on to agar medium in a petridish as described ear­lier Two or three callus masses (Nurse tissue) derived from the same plant tissue are also em­bedded directly along with the single cells in the same medium
Here the paper barrier between single cells and the nurse tissue is re­moved. Cells first begin to divide in the regions near the nurse callus indicating that the single cells closer to nurse callus in the solid medium gets the essential growth factors that are lib­erated from the callus mass. The developing colonies growing near to nurse callus also stim­ulate the division and colony formation of other cells.

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