observation of mounted pell of stomata
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1. Remove a healthy leaf from the potted plant.
2. Remove a part of the peel from the lower surface of the leaf. You can do this by folding the leaf over and gently pulling the peel apart using forceps. Keeps the peel in a watch glass containing water.
3. Put a few drops of safranin stain in a watch glass.
4. After 2-3 minutes take out the peel and place it on a clean glass slide.
5. Put a drop of glycerin over the peel and place a clean coverslip gently over it with the help of a needle.
6. Remove the excess stain and glycerin with the help of blotting paper.
7. Observe the slide under the low-power and high-power magnifications of the compound microscope.
2. Remove a part of the peel from the lower surface of the leaf. You can do this by folding the leaf over and gently pulling the peel apart using forceps. Keeps the peel in a watch glass containing water.
3. Put a few drops of safranin stain in a watch glass.
4. After 2-3 minutes take out the peel and place it on a clean glass slide.
5. Put a drop of glycerin over the peel and place a clean coverslip gently over it with the help of a needle.
6. Remove the excess stain and glycerin with the help of blotting paper.
7. Observe the slide under the low-power and high-power magnifications of the compound microscope.
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Observations:
1. The epidermal cells are visible. These are irregular in outline and have no intercellular spaces.
2. Many small pores (stomata) are seen scattered among the epidermal cells.
3. Each pore is guarded by two bean-shaped guard cells, each containing chloroplasts and a nucleus.
(a) Mounting a leaf peel (b) Epidermal layer in the peel taken form a dicot leaf showing open stomata (c) High-power magnification of stomata
4. The inner concave boundary of each guard cell is thick, whereas its outer boundary is thin.
1. The epidermal cells are visible. These are irregular in outline and have no intercellular spaces.
2. Many small pores (stomata) are seen scattered among the epidermal cells.
3. Each pore is guarded by two bean-shaped guard cells, each containing chloroplasts and a nucleus.
(a) Mounting a leaf peel (b) Epidermal layer in the peel taken form a dicot leaf showing open stomata (c) High-power magnification of stomata
4. The inner concave boundary of each guard cell is thick, whereas its outer boundary is thin.
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