Question

Observe the given sequence of nitrogenous bases on a DNA fragment and answer the following question

5- CAGAATTCTTA-3 3-GTCTTAAGAAT-5

(a) Name a restriction enzme which can recognise this DNA sequence.

(b) Write the sequence after digestion. (c) Why are the ends generated after digestion called sticky ends? (d) Name the first restriction enzyme isolated?​

Answer 1

Explanation:

"(a) EcoRI

(c) These are named sticky ends, because they form hydrogen bonds with their complementary cut parts.* "

Answer 2

Answer:

(a)EcoRI is a restriction enzme which can recognise this DNA sequence.

(c)These are named sticky ends, because they form hydrogen bonds with their complementary cut parts.

(d)HindII was the first restriction enzyme to be isolated. This enzyme was first isolated from Haemophilus influenzae Rd strain II.

Explanation:

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