on media optimization of fermentation process in fed-batch mode for the production of any licensed recombinant protein with E. coli as expression system with points below:
[Assume any E coli fermentations media composition along with at least statistically significum process variables. Assume any data with justification. Any information in public domain can be used for these
1. Control strategy applied to the fermentation for favouring higher yield of target recombinant protein
2. Screening of process variables using OFAT (One Factor At a Time) as Do technique Which DoE techniques need to be considered for optimization & validation of optimized process variables?
3. Different sensors used to monitor processes in online/Offline modes including Offline estimation of r-Protein. 4. Concept on application of digital technology like data analytics for monitoring & prediction of process consistency.
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Answer:
on media optimization of fermentation process in fed-batch mode for the production of any licensed recombinant protein with E. coli as expression system with points below:
[Assume any E coli fermentations media composition along with at least statistically significum process variables. Assume any data with justification. Any information in public domain can be used for these
1. Control strategy applied to the fermentation for favouring higher yield of target recombinant protein
2. Screening of process variables using OFAT (One Factor At a Time) as Do technique Which DoE techniques need to be considered for optimization & validation of optimized process variables?
3. Different sensors used to monitor processes in online/Offline modes including Offline estimation of r-Protein. 4. Concept on application of digital technology like data analytics for monitoring & prediction of process consistency.