one micro mole of 48 nucleotide dna was syntesized via solid phase synthesis. this sample contains salts and dna strand in diffrent length (32-48 nt) what can be the possible procefure and instruments to purify and confirm onle 48 nt dna from the mixture
Answers
«ᴀɴsᴡᴇʀ»
We have a mixture of DNA fragments ranging in length of 32 2 48 nucleotide length.
Set the agar for Aguero's gel electrophoresis is.
That's the first thing that you need to do.
You need to set the agar for Agger's gel electro forest is make wells and pour the solution to well.
So you need to make well and poor the solution two. Well. Then you need to apply And like three city.
So that's the thing that you need to do. You need to apply electricity.
The negatively charged D. N. A. Will move from Cotto to another through the gel, smaller
the fragments faster it moves So the 32 nucleotide length fragment move the
farthest And the 48 nuclei or nucleotide
lengths long remain adjusted to the wall.
The D. N. A. Sequence be visualized by adding the C. D. Um bromide.
This is a floral chrome uh Flora chrome on exposure to our exposure 24 light visible as bright orange colored and so as the DNA strand salt will not take colors.
So the bands near the well can be caught and DNA can be extracted.
It will be having only the 48 nucleotide likes