Question

Operation of a dark field microscope.​

Answer 1

Answer:

Working Principle:

In a dark-field microscope, the object is brilliantly illuminated against a dark background (Figure 4.10). ... It is a condenser with a dark-field stop, which is an opaque disc obstructing the path of light from the light source centrally, but allowing a peripheral ring of light.

Answer 2

In optical microscopy, dark-field describes an illumination technique used to enhance the contrast in unstained samples. It works by illuminating the sample with light that will not be collected by the objective lens and thus will not form part of the image.

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