Oral Questions:
1) What is the role of urease enzyme in detection of urea?
Answers
Explanation:
It plays a major role in pathogenesis of h.pylori infection and it catalyzes the conversion of urea into carbon di oxide and ammonia
The role of urease enzyme in the detection of Urea is as follows -
• We have to do a laboratory experiment for determining the role of urease in the detection of urea.
• Materials required :
Urine sample, Urease powder, Test tube, dropper , Phenol red indicator solution , 2% Na2CO3 solution, 1% Acetic acid solution.
• Procedure :
- Take 5ml of urine sample in a test tube.
- Then add few drops of fenol red solution in the previous test tube which contains the urine sample.
- Then add few drops of 2% Na2CO3 solution in the test tube which makes the colour of the solution pink or red.
- Then add few drops of 1% acetic acid solution in the test tube which will make the solution a colourless solution.
- Then add small amount of urease powder in that test tube and the colour will become again pink or pinkish red.
• Conclusion :
The again appearance of pink or pinkish red colour indicates that the urine sample has some amount of urea.