Question

Out of 50 mg/ml stock solution of plant extract, 20 μl is taken which is equivalent to 1000 μg/ml and transfer to 5 ml of broth (first tube). Other tubes were filled with 4 ml of broth and 1 ml is taken from the first tube and transferred to the second tube. Likewise the dilutions were done for concentrations 500 μg/ml, 250 μg/ml , 128 μg/ml, 64 μg/ml, 32 μg/ml, 16 μg/ml and 8 μg/ml. Each tube after dilution is inoculated with 20 μl of bacteria with turbidity of 0.5 McFarland standard.
Finally the tubes were left for 24 hours in the incubator.

Answer 1

Answer:

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Answer 2

Answer:

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