palindromic nucleotide sequence
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A palindromic sequence is a nucleic acid sequence in a double-stranded DNA or RNA molecule whereby reading in a certain direction (e.g. 5' to 3') on one strand is identical to the sequence in the same direction (e.g. 5' to 3') on the complementary strand.
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The palindrome sequence is the same front to back on both sides. Enzymes recognize sequences regardless of which side the enzyme accesses the DNA. Palindromic sequencing plays an important role in molecular biology because the DNA sequence is double-stranded and can be read to determine the palindrome. Palindromic sequences are usually 3 to 5 bases in length. The significance of this sequence is that it reads identically in both directions.
example:
3'-CTTAAG- 5'
5'-GAATTC-3'
- The top strand reads from 3' -CTTAAG -5' and the bottom strand reads 5' -GAATTC -3' .
- This palindrome sequence is recognized by the EcoR1 enzyme..
- In this case, if the DNA strands are reversed, the sequences will be exactly the same.
- The palindromic sequence also consists of a methylation site to which a methyl group can be attached to the palindromic sequence.
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