pBR322 cloning vector characteristics ?
Answers
hey mate...
here is your answer...
Plasmid pBR322 has been completely sequenced. It characterizes pBR322 in terms of its restriction sites i.e. exact length of every fragment can be calculated. These fragments can serve as DNA markers for sizing any other DNA.It can be purified with ease and so can the recombinant DNA molecules constituted within it.
There are over 40 enzymes with unique cleavage sites on the pBR322 genome. The target sites of 11 of these enzymes lie within the TcR gene, and there are sites for a further two within the promoter of that gene. There are unique sites for six enzymes within the ApR gene. Cloning can be done in pBR322 with the aid of any one of those 19 enzymes will result in insertional inactivation of either the ApR or the TcR markers. However, cloning in the other unique sites does not permit the easy selection of recombinants, because neither of the antibiotic resistance determinants is inactivated.
One of the advantages of pBR322 is that it has a high copy number.Thus an E.coli culture provies a good yield of recombinant pBR322 molecule.
Transformants are selected on the basis of their Ap resistance and then replica-plated on to Tc-containing media to identify those that are TcS. Transformants are selected on rich medium containing soluble starch and Tc. When colonized plates are flooded with an indicator solution of iodine and penicillin, â-lactamase-producing (ApR) colonies clear the indicator solution whereas ApS colonies do not. Plasmid pBR322 has been a widely used cloning vehicle. The popularity of pBR322 is a direct result of the availability of an extensive body of information on its structure and function. This in turn is increased with each new study.
pBR322 DNA is a commonly used plasmid cloning vector in E. coli.
The molecule is a double-stranded circle 4,361* base pairs in length.
pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol.
hope it helps..
Answer:
Explanation:
pBR322 Vector
View sequence details.
pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1).
The molecule is a double-stranded circle 4,361* base pairs in length (2).
pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol.