Question

Pcr assay to identify genetic variation in resistance genes

Answer 1

For the other PCR, a nucleic acid sequence of the 16S rRNA gene unique to staphylococci is amplified. The second PCR assay confirms that the bacterium amplified is a staphylococcus. The PCR amplicons are electrophorized through an agarose gel, and their sizes are determined by comparing them to standard DNA fragments.