Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V⁰= 500l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l /h. Glucose concentration in the feed solution and initial cell concentration are So = 300 g/l and X⁰ = 20 g/l, respectively. The kinetic and yield coefficients of the organism are Um= 0.2h-¹, Ks= 0.5 g/l, and Y x/s= 0.3 g dw/g glucose.
a. Determine the culture volume at t = 10 h.
b. Determine the concentration of glucose at t = 10 h at quasi-steady state.
c. Determine the concentration and total amount of cells at quasi-steady state when t = 10 h.
d. If qP = 0.05 g product/g cells h and P⁰ = 0.1 g/l, determine the product concentration in
the vessel at t = 10 h
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It was used to grow yeast cells by incrementally adding sugar during fermentation. This kept the glucose concentration low and inhibited excess alcohol formation. Another example of fed batch fermentation is the later developed penicillin production.
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