Peroxisomal acyl coa oxidase quantitative analysis
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The activation and oxidation of branched-chain FA, VLCFA, and xenobiotics at the peroxisome suggest an important role for peroxisomal ACSs in cellular metabolism. The existence of multiple ACS enzymes associated with peroxisomes suggests that each isoform has a specific function in peroxisomal metabolism.
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A simple, sensitive fluorometric method for the determination of peroxisomal fatty acyl-CoA oxidase (EC 1.3.99.3) activity has been developed. Studies of enzyme activity relative to subcellular distribution and to clofibrate induction indicate that this assay is specific for peroxisomal fatty acyl-CoA oxidase.
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