Plant tissue culture: principles and methods of preparation of culture media. Nutritional aspects of plant cell and tissue culture, growth regulators. Techniques of organ (shoot tip, root, ovary, ovules, endosperm, embryo, microspore), tissues, callus, cell-suspension cultures and protoplast cultures, and their applications. Somaclonal variation, causes and consequences; induced variation and their applications. Plant regeneration, production of haploids and methods of diplodization, importance of tissue culture into plant transformation (9 hrs)
Answers
Plant tissue culture: principles and methods of preparation of culture media. Nutritional aspects of plant cell and tissue culture, growth regulators. Techniques of organ (shoot tip, root, ovary, ovules, endosperm, embryo, microspore), tissues, callus, cell-suspension cultures and protoplast cultures, and their applications. Somaclonal variation, causes and consequences; induced variation and their applications. Plant regeneration, production of haploids and methods of diplodization, importance of tissue culture into plant transformation (9 hrs)
.Cell, Tissue, and Organ Culture
Charity Waymouth
The principal purpose of cell, tissue and organ culture is to isolate, at each level of organization, the parts from the whole organism for study in experimentally controlled environments.
It is characteristic of intact organisms that a high degree of interrelationship exists and interaction occurs between the component parts. Cultivation in vitro places cells beyond the effects of the organism as a whole and of the products of all cells other than those introduced into the culture.
Artificial environments may be designed to imitate the natural physiological one, or varied at will by the deliberate introduction of particular variables and stresses.