Plasmids are suitable vectors for gene cloning because
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Plasmids are suitable vectors
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- A foreign gene is inserted in T-DNA region of the plasmid.
- The T gene becomes inactive.
- The recombined or cloning vector is now passed into plasmid free bacterium.
- It is allowed to infect the plant or single cells in tissue culture.
- The plasmid with the desired inserted gene passes into variuos parts.
- The inserted gene expresses its effect.
- Agrobacterium tumifaciens normally infects only dicot plants.
- But one with modified nonvirulent plasmid can also cause genetic changes in monocots.
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