Question

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1) Which of the following parameters is a measure of the affinity of the enzyme for its
substrate in kinetic analysis?
A) Substrate Concentration
B) 1/2 Vmax
C) Vmax
D) KM
E) Reaction Rate

2) Which of the following is not a method for determining the structure of an enzyme?
A) Electron Paramagnetic Resonance
B) High Pressure Liquid Chromatography
C) Cryo-Electron Microscopy
D) X-ray Crystallography
E) Nuclear Magnetic Resonance

3) In which of the following situations might it be desirable to produce an enzyme in laboratory strain bacteria, rather than the original host organism
A) When the original host is a
plant with slow growth rate
B) When the original host only
produces low levels of the enzyme
C) When the enzyme needs to be
tagged and purified for study
D) All of the above
E) None of the above

4) Which of the following statements about rational design is most accurate?
A) It requires intense screening but not extra strutural / mechanistic / molecular modelling data
B) None of the above
C) It requires extra strutural / mechanistic / molecular modelling data but not intense screening
D) It requires intense screening and extra strutural / mechanistic / molecular modelling data
E) It requires neither strutural / mechanistic / molecular modelling data nor intense screening

Answer 1

Answer:

1.REATION RATE

2.HIGH LIQUID CHROMATOGRAPHY

3.ALL OF THE ABOVE

4.It requires intense screening and extra strutural / mechanistic / molecular modelling data

Explanation:

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Answer 2

Answer:

1)A) Substrate Concentration

2)D) X-ray Crystallography

3)C) When the enzyme needs to be tagged and purified for study

4)D) It requires intense screening and extra strutural / mechanistic /molecular modelling data

Explanation:

• X-ray crystallography is that the experimental science determining the atomic and molecular structure of a crystal, during which the crystalline structure causes a beam of incident X-rays to diffract into many specific directions.
• Enzymes will work best if there's plenty of substrate. because the concentration of the substrate increases, so does the speed of enzyme activity. However, the speed of enzyme activity does not increase forever.
• Rational design may be a particular strategy in protein engineering, which attempts to make improved protein molecules based on the three-dimensional structure and the relationship between structure and function, which has developed over the years as a part of protein science.

What is X-ray crystallography?

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Describe the process of X-ray crystallography.

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