please someone tell that how to stain a plants leaf to observe stomata
yaar Sahi answer Dena plz
Answers
Explanation:
Material Required
A potted plant of Bryophyllum or Tradescantia
Needles
Forceps
Watch glass
Dropper
Glass slides
A brush
Coverslips
Blotting paper
Safranin
Compound microscope
Glycerine
Procedure
Pick a healthy leaf from the potted plant
Fold the leaf to gently pull the peel apart to separate a peeled section from the lower surface of the leaf. Use the forceps to perform this step. Allow the peel to remain in a watch glass holding water for some time.
In the watch glass, stain the sample by adding some drops of safranin through a dropper.
Take the peel out after 2-3 minutes. Set it on a clear glass slide
Add a drop of glycerin on the peel. Put a clear coverslip over it gently using a needle.
Excess glycerin and stain can be removed using blotting paper
Examine the slide first under a low-power and then under a high-power magnification of a compound microscope.
Diagram
Stomata opening and closure
Observation
Visible epidermal cells. The cells in their outline are irregular with no intercellular spaces
Small openings, stomata are scattered through the epidermal cells
Guard cells are observed which have chloroplasts and nucleus
Guard cells are observed having a thin outer covering and a thick inner boundary(concave)
Guard cells control the closing and opening of the stomata.
Conclusions
Epidermal cells are found containing stomata on the lower surfaces of the leaf.
Precautions
Avoid folding the leaf too much. The peel should be snipped to a proper size
The peel should always be placed at the centre of the slide and the slides should be held from the sides.
The peel should neither be overstrained nor under strained
A brush should be used to handle the peel, otherwise would damage cells.
Glycerin should be used in order to prevent drying of the peel
Coverslip needs to be placed in such a way that air bubbles are avoided
Blotting paper can be used to remove excess stain