Question

pls solve it i need correct answers

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Answer 1

itne question kai answer todhi na koi deta hai bhai! koi seprate pucho tab batou

Answer 2

1) b)

2) b

3) a

4) d

5) b

6) b

7) b

8) d

9) a

10) d

Explanation:

• Plasmid is used as a vector to carry foreign DNA
• sal I site is present in tetracycline resistance gene in pBR 322 . so it is used to litigate a foreign DNA
• Ori site is required for replication and thus controlling the replication and copy number of plasmid.
• ethidium bromide intercalate between DNA bases and give fluorescence under UV light, therefore used to stain DNA
• 1.1) Paul Berg , H. Boyer, Annie Chang, Stanley Cohen  and they use E.coli
• 2)elution is the extraction of DNA from homogenized plant or animal tissue by washing with DNA elution buffer
• 3) Hind II is the first discovered RE
• 4)best method is the separation of DNA according to their size and molecular weight by gel electrophoresis
• 5) a palindromic nucleotide sequence is a sequence when reading in a certain direction(5' to 3') on one strand identical to the sequence in same direction(5' to 3') on other complementary strand
• 2.1)Biotechnology is integration of natural science and organisms, cells, parts there of and molecular analogues for products and services.
• 2)Downstream processing means recovery and purification of biosynthetic products after their production in upstream processing.