Question

Plz answer on basis of ONION PEEL AND CHEEK CELL EXPERIMENT
While preparing a temporary mount of cheek cells, teacher asked Rohit to pick up the stain. He had four bottles A, B, C and D containing Methylene blue, glycerine, distilled water, Canada balsam respectively. Which one should he pick?
1 point
A
B
C
D

If there is extra stain on the slide, how is it removed?
0 points
Drying stain under sunlight
Evaporation by heating.
Keeping slide under running water.
absorbing with the filter paper.

9) Given below are four operations for preparing a temporary mount of human cheek cells- (i) Taking scraping from inner side of cheek and spreading it on a clean slide. (ii) Putting a drop of glycerine on the slide. (iii) Gently cover the slides with cover slip (iv) Adding 2-3 drops of Methylene blue.. The correct sequence of this operation is
1 point
i, ii, iii, iv
iv, i, iii, ii
ii. i, iv, iii
i, iii, ii, iv

10) Why is the cover slip put on the mounted material on a slide very gently?
1 point
Avoid oozing of stain.
Avoid oozing of glycerine.
Avoid entry of air bubbles.
Avoid the crushing of mounted materials.

Answer 1

Question

While preparing a temporary mount of cheek cells, teacher asked Rohit to pick up the stain. he had four bottles A, B, C and D containing Methylene blue, glycerine, distilled water, Canada balsam respectively.

which one should he pick?

A , B, C, D

Answer

He will pick

Bottle A having methylene blue.

______________________

Question

If there is extra stain on the slide how is it removed?

1) Drying stain under sunlight

2) Evaporation by heating

3) Keeping slide under running water

4) absorbing with the filter paper.

Answer

Correct option is

3) Keeping slide under running water.

______________________

Answer 9

The correct sequence is not in the options

because

the sequence arranged in correct order will be

(i) , (iv) , (ii), (iii)

______________________

Answer 10

Correct option is

Avoiding entry of bubbles.

______________________

Answer 2

1. The stain that should be picked is A) Methylene blue.

2. If there is extra stain on the slide, it is removed by absorbing with the filter paper.

3.The correct sequence of this operation is

(i),(iv),(ii),(iii).

4. The cover slip is put on the mounted material on a slide very gently to avoid entry of air bubbles.

Explanation:

1. The stain that should be picked is A) Methylene blue.

• Cheek cell constitute the epithelial cell, RNA and DNA.
• RNA and DNA are negatively charged molecules.
• In order to stain cheek cell, a positive dye is used.
• Methylene blue is a positively charged dye.

2. If there is extra stain on the slide, it is removed by absorbing with the filter paper.

• Generally while staining extra stain comes onto the slide.
• In order to remove the extra stain use filter paper.
• The filter paper absorbs the extra stain.
• Heating or staining under the sun will destroy the cells.
• Washing with water may wash off the entire cells and damage the cells.

3.The correct sequence of this operation is (i),(iv),(ii),(iii).

• The correct sequence of the given experiment is:

1. Wash the mouth with water and disinfectant.
2. Take scraping from inner side of cheek and spreading it on a clean slide.
3. Add 2-3 drops of Methylene blue.
4. Mount the slide by adding a drop of glycerine.
5. Gently cover the slides with cover slip.

4. The cover slip is put on the mounted material on a slide very gently to avoid entry of air bubbles.

• Coverslip is put very gently to avoid the entry of the air bubbles.
• As air bubbles will interrupt with the observations under microscope.
• Air bubbles can also cause the drying out of the slide.

Refer the image below for the slide of cheek cells.

Know more:

Difference between human cheek cell and onion peel.

https://brainly.in/question/1105999

Stains used for human cheek cell and onion peel cell.

https://brainly.in/question/2334825

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